2021.10.26 14:26 prdtts Supplementing culture media with choline
Hi all, due to the global shortage, I am having to wait 6 months for some cell culture media.
I found another media with almost the same formulation.
This media is missing Choline chloride 1mg/L as compared to my ideal media.
Would it be okay for me to purchase choline chloride powder, dissolve in molecular grade water, and add to this alternative media?
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2021.10.26 14:26 TheSinisterReadings "My mum watches me sleep" by u/Milk-and-Honey
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2021.10.26 14:26 Digimad Jason Derulo onboard great organic publicity!
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2021.10.26 14:26 Endeavourwrites The Engraved White Box (Retold) - A story shared in a small local village to the world -
Hello all, I hope this does not get removed or have me banned. I posted this at the Paranormal threat but it got removed for the reason they gave as Multiple Submissions, Post Frequency, and Segmented Submissions. But I think it's more than just that and I won't argue with them at all because It's going to waste my time. So I hope you guys don't mind me sharing the story here because the original link got taken away. It got very popular in that subreddit and I kind of like the comments people gave both personal and on my threat. They are rather heartwarming so thank you. I'm sure this story would sit well in this threat instead.
What I am about to tell you is all true. Although some simplifications allow the story to flow well for readers. I live in S-Prefecture in a small community village at the base of several hills (If you can call them hills). Our location is a place not considered too rural since there is a middle grade and high school there. But these days fewer students are enrolling and thus, most classrooms on the third and fourth floor have been converted to storerooms (Mostly unused or old stuff from cultural festivals). The story begins at this school in which I am a graduate student there.
There was at one point where there is a lack of teachers for the schools and me, being begged by my parents and the town council since I took a degree in English Literature and Language, was told to apply for a position as an English teacher there which I was hesitant but I agreed to since I broke up with my girlfriend back in K-Prefecture and also couldn't find a job that I enjoy. Thus, I returned home.
The school remains as it was since I graduated about seven years ago. Four stories high, an abandoned and unused swimming pool, a gym on the second floor that holds also three used classrooms that we don't often change their numbers and so it would always display each year, despite the students going to the next education level, as Year One Class (1, 2, 3 etc). The teacher's office is also on the second floor and there were talks of moving it to the third but this didn't materialize. Coming back to a school that you once graduated from before and then teaching the juniors, have some sort of wonderful feeling to it although the antiqueness of the building gives me the creeps.
The story begins when I and another teacher Y-chan were placed in charge of the second class, Year One Class Two, during the annual sporting event. There were many events such as track & field, and badminton held during the single day and at the end of the day, we would keep all the materials like banners, flags, and whatnot at the storage levels. It was coming close to the evening, a few students still playing football in the grey, dirt field, while most students already have made their leave. I and Y-chan were heading to the fourth floor to store some cone markers. The classrooms were on the left side while extending down a long hallway to the other side was the other staircase and a ladder that gives access to the rooftop but that was chained with some big padlock. Y- chan was joking about saying that they did so because people have been jumping from the rooftop a few years back and that their ghosts at night can be seen climbing the stairs.
Now, I'm not really a believer of ghosts or if I could see any ghosts but I somehow felt that the air added by her stupid narration just gives me the creeps. We entered the middle classroom where the lights were on and two students were inside arranging some beanbags inside a box. We placed the cone markers on one side when one of the students accidentally pulled a white box. This was obvious because of one thing, a few empty boxes on the top collapsed. The students apologized but offered to clean up the mess but since it was already evening, we dismissed them. I decided to clean the mess with Y-chan instead. We had a conversation that spark interest straight to the white box which was attracting our curiosity and I think it goes as follows.
"So you say you were a student here before?" I asked.
"A year before you, if I am not wrong."
"What made you want to teach here again?"
"My mom since she belongs to the town council and since my studies are health-related, needed a coach for the school."
"Yeah and same for you as well?"
"By the way, what in hell is this?" she pulled the white box that towered right close to her waist when stood up.
"I don't know. But it looks kind of weird."
"A music box?"
There were like horse engravings, some flowers as well carved professionally and seem to be western in design. I don't think it is local at all. It was locked too and it was great since the smell of old wood was already unbearable and seeing what's inside (if it was something terrible or some curse) would be bad already. Did I mention that there was gold embodiment as well? We left the school afterwards, not thinking much of the box and parted ways home. It was then my mother pointed out to me because she believes she has some sixth sense or something. She was like seeing me with worried eyes and all that and was afraid saying that "Who is that woman following behind you?" At first, I thought it was like a prank but my mother rarely does pranks and that quickly brushes off when she started to make phone calls late at night.
"Who are you calling?" I asked.
"I am calling a priest!" She said.
"What for? Stop overreacting."
I went for a shower, the water was cold and then head straight to bed because I wanted to watch some drama on my phone but then I heard the doorbell ring. I went to answer it but find my mother with a rather old man by the entrance. He was tall, bald, and wore normal clothing unlike the usual priest style clothing but I guess that can be attributed to the importance of his visit to my home.
"I sense a dangerous and evil presence here." the man told my mother.
"What is it? What has he done?"
"I am not so sure. Let me see him."
I went back to my room as he headed up the stairs to find me inside.
"Let the priest take a look at you," my mother commanded. "He is an expert so listen to him."
I was sleepy and tired so I just did what my mother asked. He told me to bent and pull my shirt from behind and then close it back, eyes widen. He didn't tell me what it was all about but my mother did explain to me a few weeks later after the huge commotion. My mother told me that something has latched to my back. The priest saw claw-like markings on my back that only those with the vision can see and also handprints right around my neck as if that being was hugging me. It was then he decided to throw some salt at me, give me some sake and beat the crap out of me with some paper fan. Annoying as it was, I felt some sort of weight pulling me down.
He kept slapping my back with that paper fan which kept making the weight increase until it finally released and I felt no weight on my back at all.
The man then asked: "Who else has seen the box?"
"The white box!" he grew impatient and then I remembered Y-chan. He asked me for her address and I gave it to him, quickly leaving with my mother. My father who was there as well looked at me in confusion.
In the morning, I was called by the principal to not come to school and to listen to O-sensei (the priest). I didn't know why but my mother dragged me to the neighbourhood shrine. It was up at the hill which was tiring for me especially in the morning but when I got up there to see the antique shrine, I was Y-chan as well. Y-chan looked pale compared to yesterday. I don't know but I think she looked skinny as well. Her eyes showed no signs of emotions at all. Then there was the white box right in the middle of the shrine. The same box that was from the school. The priest told me that the two of us have been cursed. He said something about those who were born in the early Heisei period to parents from a different prefecture would be cursed. Essentially, any outsider who is not an original inhabitant of the village would be cursed. The priest told me to get into a small confined room with Y-chan and stay there for the whole day while they began to perform purification rituals to the box.
There was a rule that we have to follow, of course, we could not talk to each other and we must hold hands. Why? What the priest told me after the purification ritual which resulted in the box being sunk into a nearby lake, was that the box contains the corpse of a lonely priestess. These boxes were developed during the Sengoku period as a way to protect the village from large armies and that involves the sacrifice of a virgin priestess and her male companion. It is said that her power would be sufficient enough to protect the village and that the male partner was a way to protect her from evil spirits that would attack her in the afterlife. In our village, there is also the practice of holding hands and not speaking to our bride upon marriage and thus, they were trying to mimic that when they performed the purification ritual. What I was told is that the last priestess that was to be sacrificed could not have a male partner as all the young men went to war and thus, the priests tried to do the ritual without the male partner. This led to a feeling of loneliness that the priestess had and thus, she attached herself to me.
Why Y-chan was affected was another different story. She was affected because the priestess assumed that she was my female partner and thus, cursed her until she could find another male partner for herself.
When the ritual was done, we were told that both of us could not marry each other (How cruel!).
Also, I should mention the box was found in the school storage area was because that storage area was used for storing old artefacts belonging to the village as well so maybe we could have stumbled upon the Kotoribako or something. But I guess that didn't happen and I was happy that the whole ordeal was over.
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2021.10.26 14:26 VanVikas Hamas Shroomjak giving flowers to nun.
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2021.10.26 14:26 playtowinthegame_1 Any U.S. Law Schools That Don't Require a Personal Statement?
I know that in Canada there are a couple of law schools that don't require a personal statement. Just wondering if there are any U.S. law schools like that?
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2021.10.26 14:26 Sksksosos111 As a teenager, it’s considered socially acceptable to be into anyone within 2 years of your age. I’m 17, and I found the perfect 15 year old. Tips?
I’m a boy, and they are a boy.
He’s 15 and he looks 10 on the outside. He barely even looks like he went through puberty.
He doesn’t even look 5 ft tall. This is genius. I can be with someone who’s socially acceptable, but looks like an elementary schooler on the outside. It’s the closest thing I can get to a child.
He doesn’t know me as of right now. He’s in one of my classes.
Any tips on getting into a friendship and eventually getting him to change his feelings, become gay, and have us date.
That kid has to be the one. He looks 10 on the outside, and he’s not even 5 ft tall, and I get to be the one with power.
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2021.10.26 14:26 wetdirtyrat how to stop craving dick?
I sucked dick and took dick up my ass and I felt nothing besides boredom then why in the god's name I still want to do it. What do I even get out of it?
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2021.10.26 14:26 Suitable_Amphibian10 I learned today that torties can be stacked!
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2021.10.26 14:26 adamhello2 3 Day Mute on the FB group
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2021.10.26 14:26 SnooFoxes1884 Part of my collection that aren’t k-tel records
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2021.10.26 14:26 inspiredfaith I don't believe in a pre-trib rapture.
But I do believe God will protect us during the Tribulation!
Revelation 12:6says God will prepare a place for us in the wilderness. It won't be 5 star hotel accommodation, but it'll do the trick!
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2021.10.26 14:26 oh_hai_brian [WTS] 1981X by Alexandria Fragrances
2021.10.26 14:26 AliceCottonSox Would you wanna come over and play with me 😋
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2021.10.26 14:26 Stoukeer Gauss minigun question
So yesterday I bought a plan from Minerva and today I crafted to miniguns that are similar, but I have no idea which one to pick, so I ask you for help.
Both of the miniguns are quad and +50% durability. The second stars are +25% damage while aiming and +25% faster fire rate. Which one is better in your opinion?
Is quad minigun even good? 2000 rounds seems a bit excessive in my opinion. Should I try rolling for AA or Aristocrats instead? I play full health/heavy guns build.
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2021.10.26 14:26 Rodolfo_cabrita [FOR HIRE] open commissions.
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2021.10.26 14:26 garonbooth7 Advice on what to improve on.
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2021.10.26 14:26 Dima38 2v2 Sages vs Uchiha. Bloodlusted, Full Intel, Full Prep. Who wins?
2021.10.26 14:26 rachsherratt21 Cancelled my wedding and unsure what to do with my dress. Any suggestions?
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2021.10.26 14:26 TypicalExpert Selling my Opal (Base Only) any offers?
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2021.10.26 14:26 FarRelation3062 Has inflation affected your tipping habits?
2021.10.26 14:26 FreezyChan me_irl
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2021.10.26 14:26 AtypicalNiK Linkin Park & Evanescence - You'll Never Shine [Unreleased 2003] - Fake? - - - YES
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2021.10.26 14:26 thefossilfinder The invasion has begun
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2021.10.26 14:26 DataDrivenMTG Preliminary Analyses: How do you feel about the hybrid mana rule in EDH?
In case you missed it, in a previous post I discussed the hybrid mana rule and provided a survey link to get peoples' opinions on the hybrid mana rule. I'm returning today to provide some preliminary analyses for your review!
I've done a full writeup for free here, but figured I'd grab some of the highlights for discussion.